Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX)
نویسندگان
چکیده
منابع مشابه
Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX)
LAGLIDADG homing endonucleases (LHEs) are a class of rare-cleaving nucleases that possess several unique attributes for genome engineering applications. An important approach for advancing LHE technology is the generation of a library of design 'starting points' through the discovery and characterization of natural LHEs with diverse specificities. However, while identification of natural LHE pr...
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Experimental analysis and manipulation of protein-DNA interactions pose unique biophysical challenges arising from the structural and chemical homogeneity of DNA polymers. We report the use of yeast surface display for analytical and selection-based applications for the interaction between a LAGLIDADG homing endonuclease and its DNA target. Quantitative flow cytometry using oligonucleotide subs...
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Plasmodium falciparum malaria parasites invade and remodel human red blood cells (RBCs) by trafficking parasite-synthesized proteins to the RBC surface. While these proteins mediate interactions with host cells that contribute to disease pathogenesis, the infected RBC surface proteome remains poorly characterized. Here we use a novel strategy (I-SELEX) to discover high affinity aptamers that se...
متن کاملDevelopment and characterization of an aptamer binding ligand of fractalkine using domain targeted SELEX.
Fractalkine (FKN) is a unique cell surface protein with potential as a therapeutic target because of its role in inflammatory diseases and cancer. We developed an aptamer, named FKN-S2, with a dissociation constant of 3.4 ± 0.7 nM that is specific to the chemokine domain of fractalkine.
متن کامل(SELEX Collaboration)
We report a precise measurement of the D s meson lifetime. The data were taken by the SELEX experiment (E781) spectrometer using 600 GeV/c Σ, π and p beams. The measurement has been done using 918 reconstructed D s . The lifetime of the D s is measured to be 472.5±17.2±6.6 fs, using K (892)K and φπ decay modes. The lifetime ratio of D s to D 0 is 1.145 ± 0.049.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2016
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkw864